51�� receives NIH grant to investigate hearing loss mechanisms

51�� receives NIH grant to investigate hearing loss mechanisms

Mitchell Day is heading a team of OHIO scientists in an NIH-funded study aimed at uncovering the neural mechanisms behind challenges in sound localization in hearing—a crucial ability for navigating daily life.

Samantha Pelham | November 18, 2024

51�� College of Arts and Sciences Professor Mitchell Day has been awarded a significant grant of $453,000 from the National Institutes of Health (NIH) to explore the neurobiological mechanisms behind hearing loss and its impact on sound localization. This innovative research aims to shed light on how hearing impairment affects auditory perception, particularly in complex environments.

The project focuses on understanding the neural mechanisms that contribute to difficulties in sound localization—an essential skill for navigating everyday life. It will also allow for the researchers to locate where exactly in the brain this problem with auditory information is arising.

“When individuals lose their hearing, they often rely on hearing aids, which amplify sounds but do not address changes in the inner ear and the brain’s auditory processing areas,” Day said. “A lot of times, people with hearing aids can hear everything, let’s say in a crowded restaurant, however, the problem comes from the difficulty in segregating sounds based on where they are coming from. Our research seeks to investigate these changes and their implications for sound perception.”

Day goes on to explain that people with normal hearing can pinpoint the specific locations of where a sound is coming from, whereas people with hearing impairments have a hard time pinpointing the exact location of specific sounds.

Hearing loss rabbit — A look at the experimental room that will test rabbit's sound source location.

Tackling a Clinical Challenge

Hearing loss is a pressing issue, particularly among older adults. According to the , more than 700 million people (1 in every 10) will experience disabling hearing loss by 2050. To help mitigate the impacts of hearing loss, this NIH grant will fund a three-year study that utilizes an animal model to examine how hearing impairment alters auditory processing in the brain.

“Currently, we don’t know why individuals have this impairment with hearing loss. So as a neuroscientist, I’m investigating this with animal models so we can look at neural functions that are similar to that of a human’s brain,” Day said. “We’re using rabbits because they’re mammals, so they have similar auditory circuitry to humans and also have a frequency range that largely overlaps with humans.”

The research specifically targets the inferior colliculus, a critical area in the midbrain for processing information on sound location. By implanting microelectrodes into this region of the rabbit’s brain, researchers can record activity from individual neurons while the animals listen to sounds from various locations. This will help determine how hearing impairment affects the brain's ability to encode sound source location.

According to Day, neurons communicate by sending brief, large electrical impulses known as action potentials. All sensory information is encoded in the rate at which these action potentials fire.

“We can determine if a neuron encodes information about a sound source's location by recording from a neuron in the inferior colliculus and measuring its firing rate in response to a sound source,” Day explained. “If we move the sound source to different locations and observe a change in the firing rate, this suggests the neuron is sensitive to sound source location, meaning the firing rate encodes that information.”

In animals with normal hearing, an individual neuron's firing rate is strongly modulated by the location of the sound source. For instance, if the sound comes from the right, then moves to the center and to the left, the neuron's firing rate changes accordingly, sometimes even dropping to zero. This indicates that the neuron's response is influenced by sound source location. However, in hearing-impaired animals, the way their brains encode sound location may be altered. For example, the same neuron might initially respond to a sound source on the right but barely change its firing rate as the sound moves to other locations. This suggests the neuron is no longer effectively encoding information about the sound source's location.

Graduate student Olivia Barnes and Professor Mitchell Day

Graduate student Olivia Barnes and Professor Mitchell Day

Interdisciplinary Collaboration

In addition to Day’s work, this research is also supported by co-investigators Soichi Tanda from the Department of Biological Sciences and Mark Berryman from Biomedical Sciences in the Heritage College of Osteopathic Medicine. They will focus on examining cochlear tissue to assess any damage to hair cells caused by noise exposure, providing valuable insights into the anatomical aspects of hearing loss. Berryman will use the confocal microscope in the Heritage College of Osteopathic Medicine Microscopy Core to find detailed visualization of specific cellular and subcellular structures that are critical to normal hearing, as well as pathological aspects associated with hearing loss.

“Understanding how exposure to acute or chronic loud noises cause physical damage to the inner ear and compromise processing of sound signals by the brain will heighten awareness of the long-term consequences of noise-related hearing loss and ways to prevent permanent damage to one of our crucial special senses,” Berryman explained.

Graduate student Olivia Barnes is also contributing to the project by studying the effects of noise-induced hearing loss on the inferior colliculus. The research team aims to integrate undergraduate students into the project, fostering educational opportunities in biomedical research as part of the NIH grant's broader goals.

Implications for the Future

While the research has a foundational scientific focus, its implications are clinically relevant. By identifying the specific neural changes associated with hearing loss, the team hopes to pave the way for developing better interventions and treatments.

“This is just the beginning,” Day said. “Once we can pinpoint the neural basis of sound localization deficits, we can explore why these changes occur and how we might address them. We are creating the foundational research needed to ultimately find a solution to help.”

The grant was officially funded in September, and although preliminary work began earlier, the team is eager to gather data and expand their research efforts in the coming months and years.

“Hearing loss is one of the most personally, socially and economically relevant problems for all people and all ages and it presents itself in many different forms and levels of severity,” Berryman added. “While there are many known causes of hearing loss, including genetic factors, use of certain antibiotics to treat ear infections in children, exposure to loud environmental noises, chemotherapy, aging, etc., the underlying cause-effect relationships are only just beginning to be understood.”